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anti duox2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti duox2
    Anti Duox2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+duox2/DUOX2+Antibody/pm40875107-90-58-59
    Average 93 stars, based on 23 article reviews
    anti duox2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Saline:

    Article Title: Dual Oxidase 2 (Duox2) Regulates Pannexin 1-mediated ATP Release in Primary Human Airway Epithelial Cells via Changes in Intracellular pH and Not H 2 O 2 Production
    Article Snippet: Proteins were separated on a 4–20% precast Ready Gel (Bio-Rad) and blotted onto Immobilon-P membranes (Millipore, Billerica, MA). .. Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline (pH 7.4) with 0.05% Tween 20 (TTBS) for 1 h. Primary antibodies were as follows: rabbit anti-Panx1 antibody (catalog no. 488100, lot no. 1493629A, Invitrogen), used as prescribed previously ( 5 ); mouse anti-Duox1 and anti-Duox2 (catalog no. sc-393096, lot no. E2014, and catalog no.. sc-398681, lot no. G3014, Santa Cruz Biotechnology, Dallas, TX); and mouse anti-β-actin antibody (catalog no. A2228, lot no. 123M4887V, Sigma-Aldrich). ..

    SDS Page:

    Article Title: HADHB mediates 5-fluorouracil sensitivity in colorectal cancer.
    Article Snippet: Protein concentrations were determined using bicinchoninic acid (BCA) assay (Thermo Fisher Scientific, Waltham, USA) according to manufacturer’s instructions. .. Equal amounts of protein (30 μg per lane) were separated by 10% SDS-PAGE (Bio-Rad, 4561033) and transferred to PVDF membranes (Millipore, IPVH00010) using a wet transfer system (Bio-Rad) at 100 V for 90 min. Membranes were blocked with 5% non-fat milk in TBST for 1 h at room temperature before incubation with primary antibodies overnight at 4 °C: anti-DUOX2 (Santa Cruz, California, United States, 1:1000), anti-HADHB (Sino Biological, Beijing, China, 1:2000), and anti-GAPDH (Proteintech, Wuhan, China, 1:5000) as loading control. .. After washing with TBST (3 × 10 min), membranes were incubated with HRP-conjugated secondary antibodies (ZSGB-BIO, PV-6001, 1:5000) for 1 h at room temperature.

    Article Title: HADHB mediates 5-fluorouracil sensitivity in colorectal cancer
    Article Snippet: Protein concentrations were determined using bicinchoninic acid (BCA) assay (Thermo Fisher Scientific, Waltham, USA) according to manufacturer’s instructions. .. Equal amounts of protein (30 μg per lane) were separated by 10% SDS-PAGE (Bio-Rad, 4561033) and transferred to PVDF membranes (Millipore, IPVH00010) using a wet transfer system (Bio-Rad) at 100 V for 90 min. Membranes were blocked with 5% non-fat milk in TBST for 1 h at room temperature before incubation with primary antibodies overnight at 4 °C: anti-DUOX2 (Santa Cruz, California, United States, 1:1000), anti-HADHB (Sino Biological, Beijing, China, 1:2000), and anti-GAPDH (Proteintech, Wuhan, China, 1:5000) as loading control. .. After washing with TBST (3 × 10 min), membranes were incubated with HRP-conjugated secondary antibodies (ZSGB-BIO, PV-6001, 1:5000) for 1 h at room temperature.

    Incubation:

    Article Title: HADHB mediates 5-fluorouracil sensitivity in colorectal cancer.
    Article Snippet: Protein concentrations were determined using bicinchoninic acid (BCA) assay (Thermo Fisher Scientific, Waltham, USA) according to manufacturer’s instructions. .. Equal amounts of protein (30 μg per lane) were separated by 10% SDS-PAGE (Bio-Rad, 4561033) and transferred to PVDF membranes (Millipore, IPVH00010) using a wet transfer system (Bio-Rad) at 100 V for 90 min. Membranes were blocked with 5% non-fat milk in TBST for 1 h at room temperature before incubation with primary antibodies overnight at 4 °C: anti-DUOX2 (Santa Cruz, California, United States, 1:1000), anti-HADHB (Sino Biological, Beijing, China, 1:2000), and anti-GAPDH (Proteintech, Wuhan, China, 1:5000) as loading control. .. After washing with TBST (3 × 10 min), membranes were incubated with HRP-conjugated secondary antibodies (ZSGB-BIO, PV-6001, 1:5000) for 1 h at room temperature.

    Article Title: HADHB mediates 5-fluorouracil sensitivity in colorectal cancer
    Article Snippet: Protein concentrations were determined using bicinchoninic acid (BCA) assay (Thermo Fisher Scientific, Waltham, USA) according to manufacturer’s instructions. .. Equal amounts of protein (30 μg per lane) were separated by 10% SDS-PAGE (Bio-Rad, 4561033) and transferred to PVDF membranes (Millipore, IPVH00010) using a wet transfer system (Bio-Rad) at 100 V for 90 min. Membranes were blocked with 5% non-fat milk in TBST for 1 h at room temperature before incubation with primary antibodies overnight at 4 °C: anti-DUOX2 (Santa Cruz, California, United States, 1:1000), anti-HADHB (Sino Biological, Beijing, China, 1:2000), and anti-GAPDH (Proteintech, Wuhan, China, 1:5000) as loading control. .. After washing with TBST (3 × 10 min), membranes were incubated with HRP-conjugated secondary antibodies (ZSGB-BIO, PV-6001, 1:5000) for 1 h at room temperature.

    Control:

    Article Title: HADHB mediates 5-fluorouracil sensitivity in colorectal cancer.
    Article Snippet: Protein concentrations were determined using bicinchoninic acid (BCA) assay (Thermo Fisher Scientific, Waltham, USA) according to manufacturer’s instructions. .. Equal amounts of protein (30 μg per lane) were separated by 10% SDS-PAGE (Bio-Rad, 4561033) and transferred to PVDF membranes (Millipore, IPVH00010) using a wet transfer system (Bio-Rad) at 100 V for 90 min. Membranes were blocked with 5% non-fat milk in TBST for 1 h at room temperature before incubation with primary antibodies overnight at 4 °C: anti-DUOX2 (Santa Cruz, California, United States, 1:1000), anti-HADHB (Sino Biological, Beijing, China, 1:2000), and anti-GAPDH (Proteintech, Wuhan, China, 1:5000) as loading control. .. After washing with TBST (3 × 10 min), membranes were incubated with HRP-conjugated secondary antibodies (ZSGB-BIO, PV-6001, 1:5000) for 1 h at room temperature.

    Article Title: HADHB mediates 5-fluorouracil sensitivity in colorectal cancer
    Article Snippet: Protein concentrations were determined using bicinchoninic acid (BCA) assay (Thermo Fisher Scientific, Waltham, USA) according to manufacturer’s instructions. .. Equal amounts of protein (30 μg per lane) were separated by 10% SDS-PAGE (Bio-Rad, 4561033) and transferred to PVDF membranes (Millipore, IPVH00010) using a wet transfer system (Bio-Rad) at 100 V for 90 min. Membranes were blocked with 5% non-fat milk in TBST for 1 h at room temperature before incubation with primary antibodies overnight at 4 °C: anti-DUOX2 (Santa Cruz, California, United States, 1:1000), anti-HADHB (Sino Biological, Beijing, China, 1:2000), and anti-GAPDH (Proteintech, Wuhan, China, 1:5000) as loading control. .. After washing with TBST (3 × 10 min), membranes were incubated with HRP-conjugated secondary antibodies (ZSGB-BIO, PV-6001, 1:5000) for 1 h at room temperature.



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